Development of Rapid Diagnostic Reagents for Respiratory Tract Infections in Children

Kei Numazaki 1 *
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1 Division of International Infectious Diseases, Graduate School and Departments of Pediatrics, University Hospital, International University of Health and Welfare
* Corresponding Author
EUR J BASIC MED SCI, Volume 5, Issue 4, pp. 51-60. https://doi.org/10.21601/ejbms/9173
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ABSTRACT

Rapid diagnosis of respiratory tract infections is important not only from the standpoint of clinical diagnosis but also infection control. The purpose of the present study is to investigate the possibility of diagnosing with the immunochromatography for pathogens of respiratory tract. A prospective, populationbased study covering the total population less than 15 years of age in Tochigi, Japan, during the period of December 2010 to March 2013. Nasopharyngeal swabs were collected from 200 children with respiratory tract infections. RTPCR analysis was used as the reference assay. The correlation of the results between obtained by the diagnostic reagents and RT-PCR assays was investigated. Pathogens were identified in a total of 64 (32.0%) out of 200 patients by RT-PCR as follows: respiratory syncytial (RS) virus, 29; M. pneumoniae, 17; metapneumovirus, 7; adenovirus, 5; influenza A virus, 3; and influenza B virus, 3. More than 100 copies/μl of Mycoplasma pneumoniae was obtained. Minimum detection sensitivity of the immuno-chromatography in the clinical material was the 4 x 101copies/micro-liter. The correlation of the results between obtained by the diagnostic reagent for measles and RT-PCR was the positive match rate of 45.5% and negative match rate is 100%. From 79.0 to 100% of RT-PCR positive samples more than 4 x 101copies/micro-liter was detected. The importance of M. pneumoniae and RS virus in the etiology of respiratory tract infections in was confirmed. Immuno-chromatography can be applied as a simple rapid diagnostic method from the beginning of the disease in general clinical practice.
Keywords: rapid diagnosis, immuno-chromatography, RT-PCR, respiratory tract infection, virus

CITATION

Numazaki K. Development of Rapid Diagnostic Reagents for Respiratory Tract Infections in Children. Eur J Basic Med Sci. 2015;5(4):51-60. https://doi.org/10.21601/ejbms/9173

REFERENCES

  • Myint S. Recent advances in the rapid diagnosis of respiratory tract infection. Br. Med. Bull. 2002; 61: 97-114.
  • Mahony JB. Detection of respiratory viruses by molecular methods. Clin. Microbiol. Rev. 2008; 21: 716–47.
  • Jartti T, Jartti L, Peltola V, Waris M, Ruuskanen O. Identification of respiratory viruses in asymptomatic subjects: asymptomatic respiratory viral infections. Pediatr. Infect. Dis. J. 2008; 27: 1103–7.
  • van der Zalm MM, van Ewijk BE, Wilbrink B, Uiterwaal CSPM, Wolfs TFW, van der Ent CK. Respiratory pathogens in children with and without respiratory symptoms. J. Pediatr. 2009; 154: 396–400.
  • Winther B, Alper CM, Mandel EM, Doyle WJ, Hendley JO. Temporal relationships between colds, upper respiratory viruses detected by polymerase chain reaction, and otitis media in young children followed through a typical cold season. Pediatrics 2007; 119: 1069–75.
  • Liu F-C, Chen P-Y , Huang F-L , Tsai C-R , Lee C-Y , Wang L-C. Rapid diagnosis of Mycoplasma pneumoniae infection in children by polymerase chain reaction. J. Microbiol. Immunol. Infect. 2007; 40: 507-12.
  • Mubarak HS, Van De Bildt MW, Mustafa OA, Vos HW, Mukhtar MM, Groen J, El Hassan AM, Niesters HG, Ibrahim SA, Zijlstra EE, Wild TF, Osterhaus AD, De Swart RL. Serological and virological characterization of clinically diagnosed cases of measles in suburban Khartoum. J. Clin. Microbiol. 2000; 38: 987–91.
  • Hurta AC, Alexanderc R, Hibbert J, Deeda N, Barra IG. Performance of six influenza rapid tests in detecting human influenza in clinical specimens. J. Clin. Virol. 2007; 39: 132–5.
  • Nerbrand C, Jasir A, Schalén C. Are current rapid detection tests for Group A streptococci sensitive enough? Evaluation of 2 commercial kits. Scand. J. Infect. Dis. 2002; 34: 797–9.
  • Fujimoto T, Okafuji T, Okafuji T, Ito M, Nukuzuma S, Chikahira M, Nishio O. Evaluation of a bedside immunochromatographic test for detection of adenovirus in respiratory samples, by comparison to virus isolation, PCR and real-time-PCR. J. Clin. Microbiol. 2004; 42: 5489-92.
  • Hasegawa T, Asaeda A, Hamaguchi Y, Numazaki K. Development of rapid diagnostic reagent for measles. Hybridoma 2009; 28: 241-9.
  • Numazaki K. A study on the clinical application of a rapid diagnostic reagent for measles. Eur. J. Gen. Med. 2012; 9: 39-44.
  • Santibanez S, Tischer A, Heider A, Siedler A, Hengel H. Rapid replacement of endemic measles virus genotypes. J. Med. Virol. 2002; 83: 2699–708.
  • Viola B, Viktoria G, Tatsuya S, Yaeta E, Tamas M. A set of ligation-independent in vitro translation vectors for eukaryotic protein production. BMC Biotechnol. 2008; 8: 32.
  • Graves MC. Measles virus polypeptide in infected cell studied by immune precipitation and one-dimensional peptide mapping. J. Virol. 1981; 38:224-30.
  • Sato TA, Fukuda A, Sugiura A. Characterization of major structural proteins of measles virus with monoclonal antibodies. J. Gen. Virol. 1985; 66: 1397–409.
  • Hummel KB, Lowe L, Bellini WJ, Rota PA. Development of quantitative gene-specific real-time RT-PCR assays for the detection of measles virus in clinical specimens. J. Virol. Methods 2005; 132: 166-73.
  • Jin L, Feng Y, Parry R, Cui A, Lu Y. Real-time PCR and its application to mumps rapid diagnosis. J. Med. Virol. 2007; 70: 1761–7.
  • Dykewicz CA, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Infectious Diseases Society of America, American Society for Blood and Marrow Transplantation. Guidelines for preventing opportunistic infections among hematopoietic stem cell transplant recipients: focus on community respiratory virus infections. Biol. Blood Marrow Transplant. 2001; 7: 19s-22s.
  • Winchell JM, Mitchell SL. Performing your original search, RT-PCR Detection of Mycoplasma pneumoniae by real-time PCR. Methods Mol. Biol. 2013; 943: 149-58.
  • Henrickson KJ. Advances in the laboratory diagnosis of viral respiratory disease. Ped. Inf. Dis. J. 2004; 23: s6-10.
  • Erdman DD, Weinberg GA, Weinberg GA, Edwards KM, Walker FJ, Anderson BC, Winter J, González M, Anderson LJ. GeneScan reverse transcription-PCR assay for detection of six common respiratory viruses in young children hospitalized with acute respiratory illness. J. Clin. Microbiol. 2003; 41: 4298-303.
  • de-Paris F, Beck C, Machado ABMP, Paiva RM, Menezws DS, Nunes LS, Kuchenbecker R, Barth AL. Optimization of onestep duplex real-time RT-PCR for detection of influenza and respiratory syncytial virus in nasopharyngeal aspirates. J. Vitol. Methods 2012; 186: 189-92.
  • Roghmann M, Ball K, Erdman D, Lovchik J, Anderson LJ, Edelman R. Active surveillance for respiratory virus infections in adults who have undergone bone marrow and peripheral blood stem cell transplantation. Bone Marrow Transplantation 2003; 32: 1085-8.
  • Ison MG, Hayden FG. Viral infections in immunocompromised patients: what’s new with respiratory viruses? Curr. Opin. Inf. Dis. 2002; 15: 355-67.
  • Noyola DE, Demmler GJ. Effect of rapid diagnosis on management of influenza A infections. Pediatr. Infect. Dis. J. 2000; 19: 303–7.
  • Vinh DC, Newby D, Charest H, McDonald J. Evaluation of a commercial direct fluorescent-antibody assay for human metapneumovirus in respiratory specimens. J. Clin. Microbiol. 2008; 46: 1840–1.
  • Henrickson KJ, Hall CB. Diagnostic assays for respiratory syncytial virus disease. Pediatr. Infect. Dis. J. 2007; 26: S36–40.
  • Schildgen O, Muller A, Allander T, Mackay IM, Sebastian Völz S, Kupfer B, Arne Simon A. Human bocavirus: passenger or pathogen in acute respiratory tract infections? Clin. Microbiol. Rev. 2008; 21: 291-304.
  • Kesebir D, Vazquez M, Weibel C, Shapiro ED, Ferguson D, Landry ML, Kahn JS. Human bocavirus infection in young children in the United States: molecular epidemiological profile and clinical characteristics of a newly emerging respiratory virus. J. Infect. Dis. 2006; 194:1276-82.
  • Numazaki K, Chiba S, Umetsu M, Tanaka T, Yoshimura H, Kuniya Y, Miura J, Adachi N, Ukae S, Mori T, Ueda D, Hotsubo T, Sato T. Etiological agents of lower respiratory tract infections in Japanese children. In Vivo 2004; 18: 67-72.
  • Numazaki K, Umetsu M, Adachi N. Mycoplasma pneumoniae infection and its genotypical characterization in children of Hokkaido, Japan. In Vivo 2003; 17: 421-4.
  • Schwartz SB, Mitchell SL, Thurman KA, Wolff BJ, Winchell JM. Identification of P1 variants of Mycoplasma pneumoniae by use of high-resolution melt analysis. J. Clin. Microbiol. 2009; 47: 4117-20.
  • Drummond P, Clark J, Wheeler J, Galloway A, Freeman R, Cant A. Community acquired pneumonia-a prospective UK study. Arch. Dis. Child. 2000; 83: 408-12.
  • Toikka P, Juven T, Virkki R, Leinonen M, Mertsola J, Ruuskanen O. Streptococcus pneumoniae and Mycoplasma pneumoniae coinfection in community acquired pneumonia. Arch. Dis. Child. 2000; 83: 413-4.
  • Atkinson TP, Waites KB. Mycoplasma pneumoniae infections in childhood. Pediatr. Infect. Dis. J. 2014; 33: 92–4.
  • Fujino M, Yoshida N, Yamaguchi S, Hosaka N, Ota Y, Notomi T, Nakayama T. A simple method for the detection of measles virus genome by loop-mediated isothermal amplification (LAMP). J. Med. Virol. 2005; 76: 406-13.
  • Kumar S, Wang L, Fan J, Kraft A, Bose ME, Tiwari S, Van Dyke M, Haigis R, Luo T, Ghosh M, Tang H, Haghnia M, Mather EL, Weisburg WG,Henrickson KJ. Detection of 11 common viral and bacterial pathogens causing communityacquired pneumonia or sepsis in asymptomatic patients by using a multiplex reverse transcription-PCR assay with manual (enzyme hybridization) or automated (electronic microarray) detection. J. Clin. Microbiol. 2008; l46:3063–72.
  • Kikuta H, Ebihara T, Endo R, Ishiguro N, Sakata C, Ochiai S, Ishiko H, GamoI R, Sato T. Development of a rapid chromatographic immunoassay for detection of Human metapneumovirus using monoclonal antibodies against nucleoprotein of hMPV. Hybridoma 2007; 26: 17–21.
  • Helfand RF, Kim DK, Gary HE Jr, Edwards GL, Bisson GP, Papania MJ, Heath JL, Schaff DL, Bellini WJ, Redd SC, Anderson LJ. Nonclassic measles infections in an immune population exposed to measles during a college bus trip. J. Med. Virol. 1998; 56: 337-41.