Investigation of Adenoviruses in Patients with Conjunctivitis by Various Methods

Aslı Kiraz 1, Selma Gökahmetoğlu 2, Osman Özüberk 2, Ayşe Öner 3
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1 Çanakkale Onsekiz Mart University, Faculty of Medicine, Department of Microbiology and Clinical Microbiology, Çanakkale, Turkey
2 Erciyes University, Faculty of Medicine, Department of Medical Microbiology, Kayseri, Turkey
3 Erciyes University, Faculty of Medicine, Department of Ophthalmology, Kayseri, Turkey
EUR J BASIC MED SCI, Volume 3, Issue 4, pp. 83-89. https://doi.org/10.15197/sabad.2.3.16
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ABSTRACT

Adenoviruses are the most common causative agents of epidemics of community acquired and nosocomial conjunctivitis. Adenoviruses are resistant to physical and chemical factors and they can spread easily. Due to these factors, rapid and accurate diagnosis is very important in adenoviral eye infections. In this study, it was aimed to investigate adenoviruses from conjunctival swab samples of 50 patients suspected of adenoviral conjunctivitis by two different cell culture and PCR methods. Conjunctival swab samples from patients suspected of adenoviral conjunctivitis were taken between March 2012- December 2012. Adenovirus DNA was investigated by real-time PCR method in specimens. Samples were inoculated on conventional HEp-2 cell culture and HEp-2 shellvial cell culture. Virus isolation in conventional HEp-2 cell culture was confirmed with an immunofluorescence kit and a monoclonal antibody kit. Adenovirus DNA was found positive in 23 (46 %) of 50 conjunctival samples by real-time PCR. Virus isolation in conventional HEp-2 cell culture was shown in 19 (38 %) samples by immunofluorescence method and 13 (26 %) samples by monoclonal antibody method. Results obtained from Argene immunofluorescence kit were considered as positive results for conventional HEp-2 cell culture method. Shell-vial culture could be performed in 24 swab samples and 17 of samples were found positive. These 17 samples that found positive in shell-vial culture were also positive with PCR and conventional HEp-2 cell culture methods. Additionally, four samples were found positive by PCR but adenovirus could not be isolated by two cell culture methods. Two samples that were found positive with PCR and conventional HEp-2 cell culture methods were found negative by shell-vial cell culture. Considering HEp-2 cell culture confirmed with immunofluorescence method as gold standart, sensitivities and specifities were found as 100 % and 87 % respectively for PCR, 89 % and 100 % respectively for shellvial cell culture. The cell culture method is still important in adenoviral eye infections but can not provide rapid diagnosis. In adenoviral eye infections, it is considered that real-time PCR is a more useful method that provides results in the shortest period of time with high sensitivity.
Keywords: adenovirus, cell culture, conjunctivitis, PCR

CITATION

Kiraz A, Gökahmetoğlu S, Özüberk O, Öner A. Investigation of Adenoviruses in Patients with Conjunctivitis by Various Methods. Eur J Basic Med Sci. 2013;3(4):83-9. https://doi.org/10.15197/sabad.2.3.16

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