Oxidative Stress in osteoarthritis Cartilage; A Comparative Study

Alev Kural 1, Cemal Kural 2, Mahmut Ercan Çetinus 3, Ersin Erçin 2, Hatice Seval 4, Macit Koldaş 4
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1 Bakırkoy Dr. Sadi Konuk Eğitim Araştırma Hastanesi, Biyokimya Laboratuarı, İstanbul
2 Bakırkoy Dr. Sadi Konuk Eğitim Araştırma Hastanesi, Ortopedi ve Travmatoloji Kliniği, İstanbul
3 Haseki Eğitim Araştırma Hastanesi, Ortopedi ve Travmatoloji Kliniği, İstanbul
4 Haseki Eğitim Araştırma Hastanesi, Biyokimya Laboratuarı, İstanbul
EUR J BASIC MED SCI, Volume 4, Issue 4, pp. 83-88. https://doi.org/10.15197/sabad.2.4.15
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ABSTRACT

The role of increased reactive oxygen species (ROS) and/or decrease in antioxidant enzyme levels in pathogenesis of osteoarthritis (OA) is controversial. In our study we investigated antioxidant enzyme activities and oxidative stress in cartilage samples of OA patients. In our study, cartilage samples were obtained from 26 subjects (18 women, 8 man) with severe hip osteoarthritis who underwent total hip replacement and as control group; cartilage samples were obtained from 25 subjects (16 women, 9 man) who underwent hemiarthroplasty for femoral neck fracture. Superoxide dismutase (SOD), glutathione peroxidase (GP), catalase (CAT) enzyme activities and lipid hydroperoxide (LPO) levels were studied with Cayman Chemical brand kits (Ann Arbor, USA). Cartilage samples of patients with hip OA had significant higher amounts of SOD, CAT and GP antioxidant enzyme activities and oxidant LPO levels (p=0.044, p=0.028, p=0.015, p=0.001 respectively). Correlation analysis showed a weak positive relationship between SOD and LPO and between CAT and LPO (r=0.426, p= 0.01; r= 0.358 p=0.002 respectively). Oxidative stress cannot be held responsible for cartilage damage in the pathogenesis of osteoarthritis as a single factor. Studies through synovial fluid analysis with more patient numbers should be helpful to understand the pathogenesis of osteoarthritis in future research.
Keywords: cartilage, osteoarthritis, oxidative stress, antioxidant

CITATION

Kural A, Kural C, Çetinus ME, Erçin E, Seval H, Koldaş M. Oxidative Stress in osteoarthritis Cartilage; A Comparative Study. Eur J Basic Med Sci. 2014;4(4):83-8. https://doi.org/10.15197/sabad.2.4.15

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