The Role of ACE I/D Gene Mutations in The Etiology of Buergers’s Disease

Filiz Ozen 1, Nadir Kocak 2, Atilla Orhan 3, Taha Tahir Bekci 4 *
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1 Goztepe Education and Research Hospital, Department of Genetic, Istanbul
2 Konya Education and Research Hospital, Department of Genetic, Konya
3 Konya Education and Research Hospital, Department of Cardiovascular Surgery, Konya
4 Konya Education and Research Hospital, Department of Pulmonary Medicine, Konya
* Corresponding Author
EUR J BASIC MED SCI, Volume 2, Issue 1, pp. 6-11. https://doi.org/10.21601/ejbms/9168
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ABSTRACT

Thromboangiitis obliterans (TAO), which is also known as Buerger’s disease, is a disease characterized by segmental inflammation together with vasoactive phenomena in the arteries and veins of the extremities. In the present study, the relationship between angiotensin-converting enzyme (ACE) insertion/deletion (I/D) gene mutations, which is known to be involved in vasodysfunction, and TAO was determined. Because of this relationship, it was aimed to determine whether the distribution of angiotensin-converting enzyme (ACE) insertion / deletion (I / D) gene mutations in patients with Thromboangiitis obliterans (TAO) is different from healthy subjects in the control group. Eighty patients who were previously diagnosed with TAO were included in the study. The patients were determined based on Olin’s criteria. The control group consisted of 88 healthy volunteers. DNA isolation was performed by venous blood from the patients in both groups. The DNAs were amplified via polymerase chain reaction (PCR) using appropriate primers, the amplicons were eluted by running in 1% agarose gel, and defined via staining. In addition to the significant difference between the patient and control groups in terms of age and smoking due to the selection criteria (Olin’s criteria), a statistically significant difference was also determined between the groups in terms of the distribution of the ACE I/D gene polymorphism. The significant difference between groups with respect to the ACE I/D polymorphism, together with the smokinglike effects of ACE on the cardiovascular system, reveal a consequence which should not be neglected.
Keywords: polymerase chain reaction, angiotensin, mutation, genotype

CITATION

Ozen F, Kocak N, Orhan A, Bekci TT. The Role of ACE I/D Gene Mutations in The Etiology of Buergers’s Disease. Eur J Basic Med Sci. 2012;2(1):6-11. https://doi.org/10.21601/ejbms/9168

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